Instructions: This checklist is meant to serve as a general guideline for our client facilities as to the level of your skills within your nursing specialty. Please use the scale below to describe your experience/expertise in each area listed below.
Orientation and Lab Safety | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Review the laboratory’s fire safety plan. |
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Locate Personal Protective equipment and MSDS. |
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Understand Universal Precautions for microbiology. |
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Labeling & Specimen ID | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Label specimens according to institutional policies. |
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Specimen set up & incubation | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Select proper media for specimens including plated media and broth media. |
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Understand specimen collection & rejection criteria. |
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Incubate specimens properly. |
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Inoculation | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Demonstrate plate streaking for isolation & quantitative streaking for urines. |
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Quality Control | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Perform quality control procedures in accordance with institutional policies for new media, reagents, and stock culture organisms. |
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Understand documentation & actions taken when results are not in acceptable limits. |
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Gram Staining | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Practice performing Gram stains until proficient. |
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Evaluate grams stains, including sputum samples, wounds, genital samples, and positive blood cultures until proficient. |
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Evaluation of primary cultures | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Evaluate cultures to recognize what is normal flora and what is significant. |
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Evaluate throat cultures & select next course of action. |
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Evaluate urine cultures to decide when susceptibility testing is warranted. |
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Evaluate vaginal cultures to recognize what is normal flora and what is significant. |
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Evaluate stool cultures to recognize what is normal and what to process further. |
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Evaluate body fluid cultures for pathogens. |
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Evaluate wound cultures to recognize what is significant -select next course of action. |
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Evaluate respiratory cultures, including sputum cultures. Recognize normal resp. flora & significant pathogens. |
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Blood Culture processing | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Demonstrate procedure for processing positive blood cultures including subcultures, Gram stains, and proper reporting of results. |
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Primary ID of organisms | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Recognize Streptococcus species & perform tests to classify them according to the Lancefield classification scheme. |
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Recognize & perform tests to identify Staphylococcus species. |
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Identify coagulase negative Staphylococcus species. |
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Recognize and identify Haemophilus species. |
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Recognize and identify Neisseria species. |
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Recognize and perform biochemical tests to ID GNBs, including lactose fermenters, nonfermenters, and other miscellaneous GNBs. |
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Recognize and identify Campylobacter species. |
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Interpretation and Acceptance of results | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Discuss recording, reporting, and documenting results |
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Discuss which organisms are reportable to the State Health Department. |
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Serological testing | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Perform Rapid test for Group A Streptococcus, include proper labeling, QC, and reporting. |
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Affective Objectives - demonstrates honesty by: | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Maintaining strict patient confidentiality |
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Accepting control values only when within acceptable limits. |
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Performing and documenting daily & weekly maintenance procedures, preventative maintenance, temperature checks, etc. |
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Completing all procedures in adherence to laboratory SOPs, taking no shortcuts or unauthorized modifications of procedure. |
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Personal Interactive Skills - demonstrates proper professional behavior by: | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Working with co-workers in a positive manner, promoting productive workflow. |
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Refraining from making statements or actions that represent sexual, ethnic, racial, or homophobic harassment. |
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Willingly and consistently using appropriate personal safety devices when handling caustic, infectious, or hazardous materials. |
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Completing all required tasks and remaining in the work area when scheduled. |
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Being punctual whenever scheduled. |
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Adhering to current dress and appearance in the laboratory setting. |
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Cleaning the work area when leaving the laboratory, returning supplies to appropriate storage location, & disinfecting all work areas used by the student. |
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Professional Responsibility - demonstrates appropriate professional affective behavior by: | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
Correctly reporting all patient test values, as well as recognizing and correctly reporting all patient critical test values. |
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Resolving discrepancies in specimen labeling, handling, or collection before reporting patient results. |
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